iscript advanced reverse transcriptase (Bio-Rad)
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iscript advanced reverse transcriptase
Iscript Advanced Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+advanced+reverse+transcriptase/IScript+C+DNA+Synthesis+Kit/pmc12052099-186-11-15
Average 99 stars, based on 6821 article reviews
Iscript Advanced Reverse Transcriptase, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6821 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+advanced+reverse+transcriptase/IScript+C+DNA+Synthesis+Kit/pmc12052099-186-11-15
Average 99 stars, based on 6821 article reviews
iscript advanced reverse transcriptase - by Bioz Stars,
2026-10
99/100 stars
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Isolation:Article Title: Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes. Article Snippet: All QPCR analyses were performed on the StepOnePlus Real Time PCR system (ThermoFisher, Cat# 437660). .. RNA was isolated using Trizol following manufacturer’s instruction (Life Technologies, Cat. # 15596). cDNA was reverse-transcribed using Article Title: Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes Article Snippet: All QPCR analyses were performed on the StepOnePlus Real Time PCR system (ThermoFisher, Cat# 437660). .. RNA was isolated using Trizol following manufacturer’s instruction (Life Technologies, Cat. # 15596). cDNA was reverse-transcribed using Reverse Transcription:Article Title: Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes. Article Snippet: All QPCR analyses were performed on the StepOnePlus Real Time PCR system (ThermoFisher, Cat# 437660). .. RNA was isolated using Trizol following manufacturer’s instruction (Life Technologies, Cat. # 15596). cDNA was reverse-transcribed using Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis Article Snippet: .. First‐strand complementary DNA was synthesized from 1 μg total RNA, using both random hexamer and oligo(dT) 18 primers and Article Title: Deletion of the N-Terminal Domain of Yeast Eukaryotic Initiation Factor 4B Reprograms Translation and Reduces Growth in Urea Article Snippet: 0.3 ml of each gradient fraction was spiked with equal amounts of control RNA (Fluc mRNA, Trilink Biotechnologies, United States), then total RNA was extracted using the hot acid phenol-chloroform method ( ). .. First strand synthesis was performed with Article Title: Klf5 establishes bi-potential cell fate by dual regulation of ICM and TE specification genes Article Snippet: All QPCR analyses were performed on the StepOnePlus Real Time PCR system (ThermoFisher, Cat# 437660). .. RNA was isolated using Trizol following manufacturer’s instruction (Life Technologies, Cat. # 15596). cDNA was reverse-transcribed using Article Title: Monocyte-secreted Wnt reduces the efficiency of central nervous system remyelination Article Snippet: Real-time (RT) qPCR was run using BioRad Custom PrimePCR plates, as per the manufacturer’s instructions. .. Briefly, cDNA was synthesized using 5x iScript Advanced Reaction Mix and Article Title: CTC-derived pancreatic cancer models serve as research tools and are suitable for precision medicine approaches Article Snippet: iScript advanced reaction mix (iScriptTM Advanced cDNA Synthesis Kit for RT-qPCR) , BIO-RAD , 1725037. .. Article Title: Effect of Diallyl Trisulfide on TNF-α-Induced CCL2/MCP-1 Release in Genetically Different Triple-negative Breast Cancer Cells Article Snippet: .. The RNA pellet was left to dry at room temperature, resuspended in RNAase-free water, and placed on ice for 30 min. RNA purity and quantity were evaluated using Nanodrop (Thermo Fischer Scientific, Wilmington, DE, USA). cDNA synthesis and RT-PCR. Article Title: Monocyte-secreted Wnt reduces the efficiency of central nervous system remyelination. Article Snippet: Real-time quantitative polymerase chain reaction (RT-qPCR) on mouse cells Real-time (RT) qPCR was run using BioRad Custom PrimePCR plates, as per the manufacturer’s instructions. .. Briefly, cDNA was synthesized using 5x iScript Advanced Reaction Mix and Synthesized:Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis Article Snippet: .. First‐strand complementary DNA was synthesized from 1 μg total RNA, using both random hexamer and oligo(dT) 18 primers and Article Title: Monocyte-secreted Wnt reduces the efficiency of central nervous system remyelination Article Snippet: Real-time (RT) qPCR was run using BioRad Custom PrimePCR plates, as per the manufacturer’s instructions. .. Briefly, cDNA was synthesized using 5x iScript Advanced Reaction Mix and Article Title: Monocyte-secreted Wnt reduces the efficiency of central nervous system remyelination. Article Snippet: Real-time quantitative polymerase chain reaction (RT-qPCR) on mouse cells Real-time (RT) qPCR was run using BioRad Custom PrimePCR plates, as per the manufacturer’s instructions. .. Briefly, cDNA was synthesized using 5x iScript Advanced Reaction Mix and Random Hexamer:Article Title: PEAPOD repressors modulate and coordinate developmental responses to light intensity in Arabidopsis Article Snippet: .. First‐strand complementary DNA was synthesized from 1 μg total RNA, using both random hexamer and oligo(dT) 18 primers and Article Title: Deletion of the N-Terminal Domain of Yeast Eukaryotic Initiation Factor 4B Reprograms Translation and Reduces Growth in Urea Article Snippet: 0.3 ml of each gradient fraction was spiked with equal amounts of control RNA (Fluc mRNA, Trilink Biotechnologies, United States), then total RNA was extracted using the hot acid phenol-chloroform method ( ). .. First strand synthesis was performed with cDNA Synthesis:Article Title: Effect of Diallyl Trisulfide on TNF-α-Induced CCL2/MCP-1 Release in Genetically Different Triple-negative Breast Cancer Cells Article Snippet: .. The RNA pellet was left to dry at room temperature, resuspended in RNAase-free water, and placed on ice for 30 min. RNA purity and quantity were evaluated using Nanodrop (Thermo Fischer Scientific, Wilmington, DE, USA). cDNA synthesis and RT-PCR. |